Molecular Biology

Molecular Genetics

RNA processing

Posttranscriptional RNA processing is an important feature of eukaryotic cells.

Pre-mRNA processing takes place in the nucleus. RNA processing events include capping of the 5’ end on the pre-mRNA, pre-mRNA splicing to remove intronic sequences, and polyadenylation of the 3’ end of the pre-mRNA.

Capping of the 5’ end of the nascent pre-mRNA is performed soon after initiation of transcription. Cleavage, pre-mRNA splicing, and polyadenylation usually follow termination of transcription of short primary transcripts with few introns. However, introns often are spliced out of the nascent RNA before transcription of the gene is complete for large genes with multiple introns.

diagram - pre-mRNA processing : animation of RNA splicing : animation - life cycle of an mRNA : diagram - spliceosome assembly : NCBI Molecular Cell Biology : Post-transcriptional Processing of RNAs

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